HR Excellence in Science

Microscopes and data-processing workstations

 

 

Transmission Electron Microscopes

JEM-1400 JEOL

  • Accelerating voltage: 80 - 120 kV
  • Cathode - Tungsten Filament or LaB6
  • Resolution: 0.38 nm (point image)
  • Image acquisition: CMOS camera XAROSA (EMSIS BmbH) 20MP
  • Vacuum: 10-5 Pa
  • Cryo Transfer Tomography Holder 2550
  • High-tilt tomography holder up to 80 deg
  • JEOL Specimen quartet holder
  • Software for Stitching and correlative microscopy
  • Contact person: Vaněček Jiří
  • Booking

JEM–2100F JEOL

  • Accelerating voltage: 200 kV
  • Optional: 120 kV and 100 - 80 kV
  • Cathode: Schottky emission (W-ZrO)
  • Resolving power: TEM – point 0.27 nm, lattice 0.14 nm
  • Image capture: GATAN K2 Summit direct detector 3838x3710, Super-resolution 7676x7420
    • Linear mode, counting mode with drift correction
  • JEOL Phase Plate (VOLTA)
  • Tomography cryo holder Elsa 698 with temperature controller
  • Cryo-transfer workstation
  • Software for automated single particle analysis imaging
  • Motorized stage with piezoelectric movement
  • SerialEM tomography software
  • Vacuum: 10-8 Pa
  • Solarus II advanced plasma system (pumping station with plasma cleaner Ar+O2)  
  • Contact person: Bílý Tomáš Ph.D., Gardian Zdenko, Ing. Ph.D.

Scanning electron microscopes

JSM-7401F JEOL

  • Accelerating voltage: 0.1 - 30 kV
  • Cathode: Field emission (W - single crystal)
  • Resolving power: 1 nm
  • Image capture: 1280×1024×8bit (with averaging 10bit)
  • Cryo attachment: Cryo-ALTO 2000 (GATAN) allows:
    • Sample freezing (possible transfer from HPF or Plunge Freezer)
    • Freeze fracturing
    • Feeze sublimation and sputter coating Au, Pt/Pd
  • Vacuum: 10-7 Pa
  • Contact person: Vaněček Jiří
  • Booking

Apreo ThermofisherScientific

  • Accelerating voltage: 0.2 – 30 kV
  • Electron landing energy: 20 eV – 30 keV
  • Cathode: Field emission (Schottky)
  • Resolving power: 0.7nm @15kV, 0.8nm @1kV, 0.9nm @500V, STEM 0.8nm @30kV, LowVac 1.8nm @3kV
  • Image capture: up to 6144 x 4096 px
  • Vacuum: HiVac 10-2 – 10-4 Pa, LowVac 10–500 Pa
  • Stage: high tilt range +-90 degrees
  • Extension: Serial Block Face using VolumeScope, Array tomography, Stitching software (MAPS), STEM holder for 6 TEM grids, Amira visualization software
  • Contact person:Týč Jiří, RNDr. Ph.D.

Work stations for data processing

  • 64Gb/128Gb RAM
  • MIB, AMIRA
  • Booking

 

CONTACT

Biology Centre CAS
Branišovská 1160/31
370 05 České Budějovice
Data box: r84nds8

 

+420 387 775 111 (switchboard)
+420 387 775 051 (secretariat)
+420 778 468 552 (for media)

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