HR Excellence in Science

RNDr. Zdeněk Paris, Ph.D.

Position Head of Laboratory - Laboratory of RNA Biology of Protists
Molecular Parasitology
Institute of Parasitology

Contact details +420 38777 5427
PARU II, 1. patro


Iron-sulfur clusters (ISC) are part of the active site of many enzymes and are essential for cell viability. Our study is focused on de novo synthesis of ISC in mitochondrion of Trypanosoma brucei. Additionally, thanks to wonderful collaboration with Juan D Alfonzo (Ohio State University, Columbus, Ohio, USA) we are also interested in the role of the ISC pathway on tRNA modifications and editing.

Faculty of Science, University of South Bohemia

  • Master's thesis: Expression system in Leishmania tarentolae. Defended: May 2006
  • PhD. thesis:  The impact of iron-sulfur assembly on the mitochondrial tRNA import in Trypanosoma brucei. (Supervisor: Julius Lukeš; defended: September 2010.)

Total found: 29 records
Afonin D.A., Gerasimov E.S., Škodová-Sveráková I., Záhonová K., Gahura O., Albanaz A.T.S., Myšková E., Bykova A., Paris Z., Lukeš J., Opperdoes F.R., Horváth A., Zimmer S., Yurchenko V. (2024) Blast ocrithidia nonst op mit oc hondrial g enome and its expr ession ar e r emarkably insulated from nuclear codon reassignment Nucleic Acids Research 50: 3870–3885.
DOI: 10.1093/nar/gkae168

Documents to download:
Afonin_2024_NAR (pdf)
Kachale A., Pavliková Z., Nenarokova A., Roithová A., Durante I., Miletínová P., Záhonová K., Nenarokov S., Votýpka J., Horáková E., Ross R.L., Yurchenko V., Beznosková P., Paris Z., Valášek L.S., Lukeš J. (2023) Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment Nature 613: 751–758.
DOI: 10.1038/s41586-022-05584-2

Documents to download:
Kachale_2023_Nature (pdf)
Valášek L.S., Lukeš J., Paris Z. (2023) Stops making sense – For the people? Clinical and Translational Medicine 13: e1270.
DOI: 10.1002/ctm2.1270

Documents to download:
Valasek_2023_ClinTransMed (pdf)
Hegedüsová E., Maršalová V., Kulkarni S., Paris Z. (2022) Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex RNA Biology 19: 650–661.
DOI: 10.1080/15476286.2022.2067711
Dixit S., Kessler A.C., Henderson J., Pan X., Zhao R., D'Almeida G.S., Kulkarni S., Rubio M., Hegedüsová E., Ross R.L., Limbach P., Green B.D., Paris Z., Alfonzo J. (2021) Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei Nucleic Acids Research 49: 12986–12999.
DOI: 10.1093/nar/gkab1204
Kulkarni S., Rubio M., Hegedüsová E., Ross R.L., Limbach P., Alfonzo J., Paris Z. (2021) Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis Nucleic Acids Research 49: 8247–8260.
DOI: 10.1093/nar/gkab567
Mishra A., Kaur J.N., McSkimming D.I., Hegedüsová E., Dubey A.P., Ciganda M., Paris Z., Read L. (2021) Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei. Molecular Microbiology 116: 827–840.
DOI: 10.1111/mmi.14773
Paris Z. (2021) Nuclear tRNA export in trypanosomes: a journey full of twists and turns guided by tRNA modifications Parasitology 148: 1219–1222.
DOI: 10.1017/S0031182021000482
Paris Z., Svobodová M., Kachale A., Horáková E., Nenarokova A., Lukeš J. (2021) A mitochondrial cytidine deaminase is responsible for C to U editing of tRNATrp to decode the UGA codon in Trypanosoma brucei RNA Biology 14: 278–286.
DOI: 10.1080/15476286.2021.1940445

Documents to download:
Paris_2021_RNA (pdf)
Hegedüsová E., Kulkarni S., Burgman B., Alfonzo J., Paris Z. (2019) The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei Nucleic Acids Research 47: 8620–8631.
DOI: 31392978
Kessler A.C., Kulkarni S., Paulines M.J., Rubio M., Limbach P., Paris Z., Alfonzo J. (2018) Retrograde nuclear transport from the cytoplasm is required for tRNATyr maturation in T. brucei RNA Biology 15: 528–536.
DOI: 10.1080/15476286.2017.1377878
Paris Z., Alfonzo J. (2018) How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function. IUBMB Life 70: 1207–1213.
DOI: 10.1002/iub.1957
Rubio M., Gaston K., McKenney K., Fleming I., Paris Z., Limbach P., Alfonzo J. (2017) Editing and methylation at a single site by functionally interdependent activities Nature 542: 494-497.
DOI: 10.1038/nature21396
Fleming I., Paris Z., Gaston K., Gaston K., Balakrishnan R., Fredrick K., Rubio M., Alfonzo J. (2016) A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei Scientific Reports 6: 21438.
DOI: 10.1038/srep21438
Lopes R., Silveira G., Eitler R., Vidal R., Kessler A., Hinger S., Paris Z., Alfonzo J., Polycarpo C. (2016) The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr. RNA 22: 1190-1199.
DOI: 10.1261/rna.056242.116
Zíková A., Hampl V., Paris Z., Týč J., Lukeš J. (2016) Aerobic mitochondria of parasitic protists: diverse genomes and complex functions. Molecular and Biochemical Parasitology 209: 46-57.
DOI: 10.1016/j.molbiopara.2016.02.007

Documents to download:
Zikova et al. 2016 MBP (pdf)
Horáková E., Changmai P., Paris Z., Salmon D., Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal 282: 4157-4175.
DOI: 10.1111/febs.13411

Documents to download:
Horkova et al. 2015 FEBS Journal (pdf)
Sample P., Kořený L., Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P., Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research 43: 4262-4273.
DOI: 10.1093/nar/gkv286

Documents to download:
Sample_et_al_NAR_2015 (pdf)
Verner Z., Basu S., Benz C., Dixit S., Dobáková E., Faktorová D., Hashimi H., Horáková E., Huang Z., Paris Z., Pena P., Ridlon L., Týč J., Wildridge D., Zíková A., Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology 315: 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001

Documents to download:
Verner-IRCMB-2015 (pdf)
Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S., Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA 19: 649-658.

Documents to download:
2013_Paris_RNA_649 (pdf)

More publications


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