HR Excellence in Science

RNDr. Eva Horáková, Ph.D.

Position Research Scientist - Laboratory of Molecular Biology of Protists
Molecular Parasitology
Institute of Parasitology

Contact details +420 387775481
PARU II, 1. patro





Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic

Ph.D. In Molecular Biology

Thesis :“Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics.“

supervisor: Prof. Julius Lukes, email:


Faculty of Science, Masaryk University, Brno, Czech Republic

M.S. In Molecular Biology and Genetics

Thesis: „Typization of HLA antigens in CVID patients“

supervisor: MUDr. Tomas Freiberger Ph.D., email:




Postdoctoral Fellow, Laboratory of Molecular Parasitology, Institute of Parasitology, Biology Centre, ASCR, v.v.i.

„Posttranscriptional modifications of mitochondrial and cytosolic tRNAs in Trypanosoma brucei

2009 – 2010

Postdoctoral Fellow,  McMaster laboratory, Department of Medical Genetics, Vancouver Coastal Health Research Institute,

University of British Columbia, Jack Bell Research Centre

“Proteomic Profiling of Host-Pathogen interaction: The Macrophage response to Leishmania major” Prof. Robert W. McMaster (

2006-2008 Maternity leave


Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic.

Research Scientist in Laboratory of Molecular Parasitology

2003 6 month research stay in Prof. Kenneth Stuart laboratory, Seattle, Biomedical Research Institute, Seattle, Washington, USA

2002 6 month research stay in Dr. Rob Benne laboratory, Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, supported by EURESCO Exchange Grant given to E.V.


Department of Imunogenetics, Research Institute of Child Health, Brno, Czech Republic bachelor

Student of Molecular Biology and Genetics



            Notable awards:

2007, Otto Wichterle Premium for young scientific team, AS CR

2005, honored PhD thesis “Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics”

2001, honored student grant “RNA interference in Trypanosoma brucei



“Grant Writing for Funding and Research Development” University of British Columbia

             “Mass Spectrometry in Life Sciences,” University of British Columbia

“Proactive Communication & Effective Temwork Workshop” MITACS Skills

Training&Entrepreneurship Program (STEP)



2010 Melanie van Soeren: Characterization of the Leishmania protein GP63 using site direct mutagenesis



Wide range of general lab experience, focusing on molecular biology and proteomics:

Culture work (Leishmania ssp, Trypanosoma ssp.), Culture manipulation, Transfection studies, Macrophage infection studies, THP-1 cell cultivation, Peritoneal macrophage cell harvest.

Isolation of amastigotes from spleen/lesion, axenic cultivation of amastigotes

Leishmaniainfection rate. Limited Dilution assay. Spleen/Liver smears (H/E staining)

Viability assays (MTS assay, Alamar Blue assay)

Mass spectrometry sample preparation. Operating ABI 4800 mass spec, Protein Pilot, Statistical analysis of proteomics data.

Site-direct mutagenesis

Gelatin zymography

FACS analysis.

Isolation of DNA, RNA and mitochondrial vesicles

gel electrophoresis, SDS-PAGE,, 2D gel electrophoresis

Western and Northern blotting

Radiolabelling, Imunochemical staining

RNA interference of Trypanosoma brucei

immunofluorescent microscopy

PCR, RT-PCR, Quantitative RT-PCR, poisoned primer extension

in vitro editing

measurement of mitochondrial membrane potential

measurement of respiration rate

histochemical staining of protein complexes

NADH dehydrogenase, cytochrome creductase, and oxidase activity assays


Total found: 20 records
Kachale A., Pavliková Z., Nenarokova A., Roithová A., Durante I., Miletínová P., Záhonová K., Nenarokov S., Votýpka J., Horáková E., Ross R.L., Yurchenko V., Beznosková P., Paris Z., Valášek L.S., Lukeš J. (2023) Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment Nature 613: 751–758.
DOI: 10.1038/s41586-022-05584-2

Documents to download:
Kachale_2023_Nature (pdf)
Horáková E., Lecordier L., Cunha P., Sobotka R., Changmai P., Langedijk C.J.M., Abbeele J.V.D., Vanhollebeke B., Lukeš J. (2022) Heme-deficient metabolism and impaired cellular differentiation as an evolutionary trade-off for human infectivity in Trypanosoma brucei gambiense Nature Communications 13: 7075 .
DOI: 10.1038/s41467-022-34501-4

Documents to download:
Horakova_2022_NatComm (pdf)
Kořený L., Oborník M., Horáková E., Waller R., Lukeš J. (2022) The convoluted history of haem biosynthesis Biological Reviews of the Cambridge Philosophical Society 97: 141–162.
DOI: 10.1111/brv.1279

Documents to download:
Koreny_2022_Biol-Reviews (pdf)
Paris Z., Svobodová M., Kachale A., Horáková E., Nenarokova A., Lukeš J. (2021) A mitochondrial cytidine deaminase is responsible for C to U editing of tRNATrp to decode the UGA codon in Trypanosoma brucei RNA Biology 14: 278–286.
DOI: 10.1080/15476286.2021.1940445

Documents to download:
Paris_2021_RNA (pdf)
Horáková E., Faktorová D., Kraeva N., Kaur B., Van Den Abbeele J., Yurchenko V., Lukeš J. (2020) Catalase compromises the development of the insect and mammalian stages of Trypanosoma brucei FEBS Journal 287: 964–977.
DOI: 10.1111/febs.15083

Documents to download:
Horakova_2020_FEBSJournal (pdf)
Podešvová L., Leštinová T., Horáková E., Lukeš J., Volf P., Yurchenko V. (2020) Suicidal Leishmania Pathogens 9: 79.
DOI: 10.3390/pathogens9020079

Documents to download:
Podesvova_2020_Pathogens (pdf)
Bianchi C., Kostygov A., Kraeva N., Záhonová K., Horáková E., Sobotka R., Lukeš J., Yurchenko V. (2019) An enigmatic catalase of Blastocrithidia. Molecular and Biochemical Parasitology 232: 111199.
DOI: 10.1016/j.molbiopara.2019.111199

Documents to download:
Bianchi_Molecular & Biochemical Parasitology_2019 (pdf)
Haindrich A., Boudová M., Vancová M., Pena Diaz C., Horáková E., Lukeš J. (2017) The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone Molecular and Biochemical Parasitology 214: 47-51.
DOI: 10.1016/j.molbiopara.2017.03.009

Documents to download:
Haindrich et al. 2017 MBP (pdf)
Horáková E., Changmai P., Vancová M., Sobotka R., Van Den Abbeele J., Vanhollebeke B., Lukeš J. (2017) The Trypanosoma brucei TbHrg protein is a heme transporter involved in regulation of stage-specific morphological transitions The Journal of Biological Chemistry 292: 6998-7010.
DOI: 10.1074/jbc.M116.762997

Documents to download:
Horakova et al. 2017 (pdf)
Kraeva N., Horáková E., Kostygov A., Kořený L., Butenko A., Yurchenko V., Lukeš J. (2017) Catalase in Leishmaniinae: With me or against me? Infection, Genetics and Evolution 50: 121-127.
DOI: 10.1016/j.meegid.2016.06.054

Documents to download:
Kraeva et al. 2017 JBCh (pdf)
Basu S., Horáková E., Lukeš J. (2016) Iron-associated biology of Trypanosoma brucei. Biochimica et Biophysica Acta 1860: 368-370.
DOI: 10.1016/j.bbagen.2015.10.027

Documents to download:
Basu et al. 2016 B et B Acta (pdf)
Horáková E., Changmai P., Paris Z., Salmon D., Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal 282: 4157-4175.
DOI: 10.1111/febs.13411

Documents to download:
Horkova et al. 2015 FEBS Journal (pdf)
Sample P., Kořený L., Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P., Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research 43: 4262-4273.
DOI: 10.1093/nar/gkv286

Documents to download:
Sample_et_al_NAR_2015 (pdf)
Verner Z., Basu S., Benz C., Dixit S., Dobáková E., Faktorová D., Hashimi H., Horáková E., Huang Z., Paris Z., Pena P., Ridlon L., Týč J., Wildridge D., Zíková A., Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology 315: 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001

Documents to download:
Verner-IRCMB-2015 (pdf)
Kovářová J., Horáková E., Changmai P., Vancová M., Lukeš J. (2014) Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei Eukaryotic Cell 13: 353-362.
DOI: 10.1128/EC.00235-13

Documents to download:
Kovarova-EC-2014 (pdf)
Changmai P., Horáková E., Long S., Černotíková-Stříbrná E., Mcdonald L., Bontempi E., Lukeš J. (2013) Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei Molecular microbiology 89: 135-151.
DOI: 10.1111/mmi.12264

Documents to download:
2013_Changmai_MM_135 (pdf)
Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S., Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA 19: 649-658.

Documents to download:
2013_Paris_RNA_649 (pdf)
Šmíd O., Horáková E., Vilímová V., Hrdý I., Cammack R., Horváth A., Lukeš J., Tachezy J. (2006) Knock-downs of mitochondrial iron-sulfur cluster assembly proteins IscS and IscU down-regulate the active mitochondrion of procyclic Trypanosoma brucei. Journal of Biological Chemistry 281: 28679-28686.
DOI: 10.1074/jbc.M513781200

Documents to download:
Smid et al. 2006 JBCh (pdf)
Zíková A., Horáková E., Jirků M., Dunajčíková P., Lukeš J. (2006) The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei. Molecular and Biochemical Parasitology 149: 65-73.
DOI: 10.1016/j.molbiopara.2006.04.007

Documents to download:
Zikova et al. 2006 MBP (pdf)
Horváth A., Horáková E., Dunajčíková P., Verner Z., Pravdová E., Šlapetová I., Cuninková Ľ., Lukeš J. (2005) Down-regulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei. Molecular Microbiology 58: 116-130.
DOI: 10.1111/j.1365-2958.2005.04813.x

Documents to download:
Horvath et al. 2005 MM (pdf)


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