HR Excellence in Science

prof. Ing. Miroslav Oborník, Ph.D.

Position Head of Laboratory - Laboratory of Evolutionary Protistology
Molecular Parasitology
Institute of Parasitology

Contact details +420 387775464
obornik@paru.cas.cz
236

Others

Research interests

Evolution of protists and related algae.


 

Education and employment

  • 1985-1991: Student Ing. (MSc) , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, specialization "Genetic Engineering and Plant Breeding"
  • 1991: Fellowship , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, Dept. of Plant Production, Division of Plant Protection
  • 1991-1995: PhD student , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
  • 10/1995 - 12/1996: Civil service
  • 1998 - 2000: Assistant Professor , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
  • 1998 - : Research scientist , Institute of Parasitology, Academy of Sciences of the Czech Republic, Division of Molecular Parasitology
  • 2000 - : Research scientist, head of the laboratory , Institute of Parasitology (IP), Academy of Sciences of the Czech Republic, and University of South Bohemia (USB), Faculty of Biological Sciences - Laboratory of Molecular Taxonomy (joint laboratory of IP and USB)
  • 2005-2008 Assistent Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice
  • 2008- present: Senior Research Scientist, Biology Centre ASCR, Institute of Parasitology
  • 2008- present - Associate Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice

Foreign programs

  • May - August 1993: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
  • December 1994 - September 1995: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
  • April - May 1997: University of Horticulture and Food Industry, Budapest, Hungary
  • November 2002 - November 2003: University of British Columbia, Department of Botany, Vancouver, Canada(Dr. B.R. Green)

Research priorities

MO is currently working on the evolution of secondary endosymbiosis. Many unicellular photoautotrophic eukaryotes (such as diatoms, heterokonts, cryptophytes, haptophytes, euglenoids) possess complex photosynthetic organelles that evolved during the process of secondary endosymbiogenesis. Such secondary (or complex) plastids are surrounded by three or four membranes. Nuclear-encoded proteins are postraslationally imported into the complex plastid via secretory pathway. We have participated on the annotation of the genome of diatom Thalassiosira pseudonana , particularly on the search of nuclear-encoded plastid targeted proteins, their in silico localization and phylogeny.
Complex plastid is also present in apicomplexan parasites, however, it is not photosynthetic in this case. The size of the apicomplexan plastid genome is extremely small (35 kb) thus offering a limited number of genes for phylogenetic analysis. Moreover, the sequences of apicoplast genes are highly AT rich and rapidly evolving. Although it is evident that the apicoplast and its genome is plastid-derived, our detailed phylogenetic analysis of amino acid and nucleotide sequences of selected apicoplast ribosomal protein genes rpl2 , rpl14 and rps12 show their possible mitochondrial origin. Based on our results, a hypothesis is proposed that apicoplasts might contain both plastid and mitochondrial genes, thus constituting a hybrid assembly.
MO is also involved in phylogenetic projets concerning molecular phylogeny of protozoa, e.g. microsporidia, apicomplexan parasites and kinteoplastids.

Hobby


Teaching Introduction to Bioinformatics
Faculty of Science, University of South Bohemia

Introduction to Bioinformatics exercises
Faculty of Science, University of South Bohemia

Molecular Taxonomy
Faculty of Agriculture, University of South Bohemia

Total found: 101 records
Oborník M. (2021) Enigmatic Evolutionary History of Porphobilinogen Deaminase in Eukaryotic Phototrophs Biology-Basel 10: 386.
DOI: 10.3390/biology10050386
Oborník M., Dorrell R.G., Tikhonenkov D.V. (2021) Editorial: Mixotrophic, Secondary Heterotrophic, and Parasitic Algae Frontiers in Plant Science 12: 798555.
DOI: 10.3389/fpls.2021.798555
Oborník M., Füssy Z. (2021) Evolutionary and Molecular Aspects of Plastid Endosymbioses Biomolecules 11: 1694.
DOI: 10.3390/biom11111694
Richtová J., Sheiner L., Gruber A., Yang S., Striepen B., Kořený L., Oborník M. (2021) Using Diatom and Apicomplexan Model to Study the Heme Pathway of Chromera velia International Journal of Molecular Sciences 22: 6495.
DOI: 10.3390/ijms22126495
Turnšek J., Brunson J.K., Viedma M.D.P.M., Deerinck T.J., Horák A., Oborník M., Bielinski V.A., Allen A.E. (2021) Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway eLife 10: e52770.
DOI: 10.7554/eLife.52770
Füssy Z., Záhonová K., Tomčala A., Krajčovič J., Yurchenko V., Oborník M., Eliáš M. (2020) The Cryptic Plastid of Euglena longa Defines a New Type of Nonphotosynthetic Plastid Organelle mSphere 5: e00675-20.
DOI: 10.1128/mSphere.00675-20
Gruber A., McKay C., Rocap G., Oborník M. (2020) Comparison of different versions of SignalP and TargetP for diatom plastid protein predictions with ASAFind Matters_ISSN_2297-8240 2020: e202005000001.


Documents to download:
Gruber_2020_Matters (pdf)
Horák A., Allen A.E., Oborník M. (2020) Common origin of ornithine–urea cycle in opisthokonts and stramenopiles Scientific Reports 10: 16687.
DOI: 10.1038/s41598-020-73715-8
Oborník M. (2020) Photoparasitism as an Intermediate State in theEvolution of Apicomplexan Parasites Trends in Parasitology 36: 727-734.
DOI: 10.1016/j.pt.2020.06.002
Sato S., Nanjappa D., Dorrell R.G., Vieira F.R.J., Kazamia E., Tirichine L., Veluchamy A., Heilig R., Aury J.M., Jaillon O., Wincker P., Füssy Z., Oborník M., Muñoz-Gómez S.A., Mann D., Bowler C., Zingone A. (2020) Genome-enabled Phylogenetic and Functional Reconstruction of an Araphid Pennate Diatom Plagiostriata Sp. CCMP470, Previously Assigned as a Radial Centric Diatom, and Its Bacterial Commensal Scientific Reports 10: 9449.
DOI: 10.1038/s41598-020-65941-x
Tomčala A., Michálek J., Schneedorferová I., Füssy Z., Gruber A., Vancová M., Oborník M. (2020) Fatty Acid Biosynthesis in Chromerids Biomolecules 10: 1102.
DOI: 10.3390/biom10081102
Tomečková L., Tomčala A., Oborník M., Hampl V. (2020) The Lipid Composition of Euglena gracilis Middle Plastid Membrane Resembles That of Primary Plastid Envelopes Plant Physiology 184: 2052–2063.
DOI: 10.1104/pp.20.00505
Cihlář J., Füssy Z., Oborník M. (2019) Evolution of tetrapyrrole pathway in eukaryotic phototrophs Advances in Botanical Research 90: 273–309.
DOI: 10.1016/bs.abr.2018.12.003
Coale T.H., Moosburner M., Horák A., Oborník M., Barbeau K.A., Allen A.E. (2019) Reduction-dependent siderophore assimilation in a model pennate diatom Proceedings of the National Academy of Sciences of United States of America 116: 23609-23617.
DOI: 10.1073/pnas.1907234116
Füssy Z., Faitová T., Oborník M. (2019) Subcellular Compartments Interplay for Carbon and Nitrogen Allocation in Chromera velia and Vitrella brassicaformis. Genome biology and evolution. 11: 1765-1779.
DOI: 10.1093/gbe/evz123
Jirsová D., Füssy Z., Richtová J., Gruber A., Oborník M. (2019) Morphology, ultrastructure, and mitochondrial genome of the marine non-photosynthetic bicosoecid Cafileria marina gen. et sp. nov. Microorganisms 7: 240.
DOI: 10.3390/microorganisms7080240
Oborník M. (2019) In the beginning was the word: How terminology drives our understanding of endosymbiotic organelles Microbial Cell 6: 134–141.
DOI: 10.15698/mic2019.02.669
Oborník M. (2019) Gurmánův pokrm zapomnění Vesmír 98: 112–114.
Oborník M. (2019) Endosymbiotic Evolution of Algae, Secondary Heterotrophy and Parasitism Biomolecules 9: 266.
DOI: 10.3390/biom9070266
Oborník M. (2019) Evoluce parazitických výtrusovců z fotosyntetického předka Živa 6: 287–289.

More publications

CONTACT

Biology Centre CAS
Branišovská 1160/31
370 05 České Budějovice
Data box: r84nds8

 

+420 387 775 111 (switchboard)
+420 387 775 051 (secretariat)
+420 778 468 552 (for media)

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