HR Excellence in Science

prof. RNDr. Libor Grubhoffer, CSc., Hon. D.Sc., dr. h. c.

Position 1 BC Director
Biology Centre CAS

Position 2 Head of Laboratory - Laboratory of Molecular Ecology of Vectors and Pathogens
Tick-Borne Diseases
Institute of Parasitology

Contact details +420 387775050, +420 387775456
liborex@paru.cas.cz
libor.grubhoffer@bc.cas.cz
212, 312

Others

Research interests

  • molecular and cellular factors involved in the mechanism of pathogen transmission by ticks and of the tick immunity
  • Lyme borreliosis spirochaetes and tick-borne encephalitis virus with respect to protein-carbohydrate interactions, and their interaction with the inner environment of the tick Ixodes ricinus

University Education

  • 1976-1981: Department of Biochemistry, Faculty of Science, Charles University, Prague, the Czech Republic. Area of study: biochemistry of lectins, and their biological significance at the cellular level (supervisor Professor Jan Kocourek). Isolation and characterization of the lectins from the seeds of Vicia villosa.
  • 1982: RNDr. (equivalent to M.Sc.) biochemistry, Faculty of Science, Charles University, Prague, the Czech Republic.
  • 1982-1983: Research Assistant in the Laboratory of Chemical Mutagenesis at the Research Institute of Organic Syntheses, Pardubice, Czech Republic.

Postgraduate Study

  • 1983-1986: Ph.D. Research on the antigenic structures of mumps virus (Paramyxoviridae) with the respect to their physico-chemical, immunochemical, and biological properties thesis under the guidence of Professor Dimitrij Slonim in the Department of Preparative Virology at the Institute of Sera and Vaccines, Prague, Czech Republic
  • 1986: Research Associate in the Laboratory of Arboviruses at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice, the Czech Republic
  • 1987: C.Sc. degree (equivalent to Ph.D.) in microbiology/virology. Thesis entitled "The study of antigenic structures of mumps virus and their possibilities in the practice employment" in the Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, the Czech Republic.

Employment History

  • 1987: Junior research scientist in the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1990-1994: Secretary for Science at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1991: Appointed Head of the Laboratory of Molecular Ecology of Parasites at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1993: Appointed assistant Professor in Biochemistry, Faculty of Biological Sciences, the University of South Bohemia, Česke Budějovice, the Czech republic.
  • 1994-2002: Director of the Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice.
  • 1997: Appointed associated Professor in Molecular and Cellular Biology, Faculty of Biological Sciences, the University of South Bohemia, Česke Budějovice. Teaches folowing courses: Biochemistry, Biochemistry and molecular biology of parasites, General Virology.
  • 2001: Appointed Professor in Molecular and Cellular Biology and Genetics
  • 2001-2004: Vice-rector of the University of Southern Bohemia for international relations.
  • 2002-: Chairman of the Scientific Council of the Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice.
  • 2002-: Czech Republic representative in the Central and Eastern European Countries (CEEC) forum at the International Consortium on Ticks and Tick-borne Diseases (ICTTD-2/3).
  • 2003-: Chairman of the Biomedical and molecular biological sciences panel #5 of the Grant Agency of the Academy of Sciences of the Czech Republic.
  • 2003-: Executive chairman of the National Committee of the International Union of Biological Sciences.
  • 2003-: Member of the standing committee on a D.Sc. degree defence in ´Ecological Biology´.
  • 2003-: Vice-chairman of the Committee for evaluation of the institutional research plans and results of institutes of the Academy o Sciences of the Czech Republic in Life sciences and Chemistry.

Scientific Background, Research Experience

Biochemistry and molecular biology of disease vectors; molecular factors in mechanisms of pathogens transmission with the respect to the role of protein-carbohydrate as well as other non-covalent interactions, immune reactions in arthropods. At present, he is concerned with the investigation of structural and functional components of Borrelia burgdorfferi and B. recurrentis spirochetes. He is also interested in tick-borne encephalitis virus, its virulence, separation, purification, isolation of the envelope glycoprotein E, and in the study on structural properties of this antigen. He also deals with the interaction of borreliae and TBE virus with internal environment of ixodid ticks. The other domain of his interest is the immunity of invertebrates and molecular biological factors influencing it. He has worked on the isolation and functional characterization of structures important for physiology of recognition and defense reaction in invertebrates. Till now, he has been a principal investigator of 4 grants of GA ASCR, 3 grants of GA CR, 3 grants of the Ministry of Education, one of those is VS 96066 ("Strengthening of Research at Universities"), and a co-principal investigator of two internatinal projects (IAAE; Fogarty Foundation/N.I.H.). A joint research project with NRC-IBS Ottawa within S&T Cooperation/Canada´(2003-2006).

Long-term Study Stays Abroad and International Collaborations

1989 University of Uppsala (Prof. K. Soderhall), 1992-1993, 1995 University of Arizona (Prof. M. Wells, Prof. J.Law). Develops a tight collaboration with the University of Arizona (within Biomedical Research Abroad: Vistas Open! programme), the University of Glasgow (1997-Prof. I. Maudlin; 2000-Dr. R. A. Smith: work placement of undergraduate students, Socrates/Erasmus), Karel Grote Hogeschol in Antwerpen (student mobility within Erasmus/Socrates, teaching a class on Biochemistry of Parasites). 2003 Georgia Southern University, Statesboro (USA), Institute of Parasitology and Arthropodology, (Prof. J. H. Oliver, Jr.) within NATO Senior Research Fellowship on molecular factors in tick-borrelia interactions.

Published a total of 70 scientific articles, 62 papers at conferences and 16 invited lectures. Member of the editorial board of Folia Parasitologica and of several scientific societies, and scienticic counciles of the University of South Bohemia in České Budějovice (USB), the Institute of Entomology AS CR, Institute of Plant Molecular Biology AS CR, Institute of Landscape Ecology AS CR, the Faculty of Biological Sciences USB, and the Institute of Physical Biology USB.


Teaching Biochemistry
Faculty of Science, University of South Bohemia

Basic Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Biochemistry I
Faculty of Science, University of South Bohemia

Biochemistry 1
Faculty of Science, University of South Bohemia

Biochemistry 2
Faculty of Science, University of South Bohemia

Biological Chemistry - Bachelor Seminar
Faculty of Science, University of South Bohemia

Developmental and Comparative Biochemistry (for Biological Chemistry)
Faculty of Science, University of South Bohemia

Glycobiochemistry (for Biological Chemistry)
Faculty of Science, University of South Bohemia

Laboratory practise from Biochemistry
Faculty of Science, University of South Bohemia

Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Advanced Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Seminar in Advanced Biological Chemistry
Faculty of Science, University of South Bohemia

Seminar in Advanced Biological Chemistry II
Faculty of Science, University of South Bohemia

Total found: 138 records
Hrnková J., Schneiderová I., Golovchenko M., Grubhoffer L., Rudenko N., Černý J. (2021) Role of Zoo-Housed Animals in the Ecology of Ticks and Tick-Borne Pathogens—A Review Pathogens 10: 210.
DOI: 10.3390/pathogens10020210
Strnad M., Oh Y.J., Vancová M., Hain L., Salo J., Grubhoffer L., Nebesářová J., Hytönen J., Hinterdorfer P., Rego R. O. M. (2021) Nanomechanical mechanisms of Lyme disease spirochete motility enhancement in extracellular matrix Communications Biology 4: 268 .
DOI: 10.1038/s42003-021-01783-1
Černý J., Lynn G., Hrnková J., Golovchenko M., Rudenko N., Grubhoffer L. (2020) Management Options forIxodes ricinus-AssociatedPathogens: A Review of Prevention Strategies International Journal of Environmental Researchand Public Health 17: 1830.
DOI: 10.3390/ijerph17061830
Helmová R., Hönig V., Tykalová H., Palus M., Bell-Sakyi L., Grubhoffer L. (2020) Tick-borne encephalitis virus adaptation in different host environments and existence of quasispecies Viruses 12: 902.
DOI: 10.3390/v12080902
Kaufman F., Dostálková A., Pekárek L., Thank T.D., Kapisheva M., Hadravová R., Bednárová L., Novotný R., Křížová I., Černý J., Grubhoffer L., Ruml T., Hrabal R., Rumlová M. (2020) Characterization and in vitro assembly of tick‐borne encephalitis virus C protein FEBS Letters 594: 1989-2004.
DOI: 10.1002/1873-3468.13857
Kotsarenko K., Věchtová P., Hammerová Z., Langová N., Malinovská L., Wimmerová M., Štěrba J., Grubhoffer L. (2020) Newly identified DNA methyltransferases of Ixodes ricinus ticks Ticks and Tick-borne Diseases 11: 101348.
DOI: 10.1016/j.ttbdis.2019.101348
Kotsarenko K., Věchtová P., Lieskovská J., Fussy Z., Cabral-de-Mello D.C., Rego R. O. M., Alberdi P., Collins M., Bell-Sakyi L., Štěrba J., Grubhoffer L. (2020) Karyotype changes in long-term cultured tick cell lines Scientific Reports 10: 13443.
DOI: 10.1038/s41598-020-70330-5
Lattová E., Straková P., Pokorná Formanová P., Grubhoffer L., Bell-Sakyi L., Zdráhal Z., Palus M., Růžek D. (2020) Comprehensive N-glycosylation mapping of envelope glycoprotein from tick-borne encephalitis virus grown in human and tick cells Scientific Reports 10: 13204.
DOI: 10.1038/s41598-020-70082-2
Rusanov A.L., Stepanov A.A., Zgoda V.G., Kaysheva A.L., Selinger M., Mašková H., Loginov D., Štěrba J., Grubhoffer L., Luzgina N.G. (2020) Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus. Data in Brief 28: 105029.
DOI: 10.1016/j.dib.2019.105029
Strnad M., Grubhoffer L., Rego R. O. M. (2020) Novel targets and strategies to combat borreliosis Applied Microbiology and Biotechnology 104: 1915-1925.
DOI: 10.1007/s00253-020-10375-8
Vancová M., Bílý T., Šimo L., Touš J., Horodyský P., Růžek D., Novobilský A., Salat J., Strnad M., Sonenshine D.E., Grubhoffer L., Nebesářová J. (2020) Three-dimensional reconstruction of the feeding apparatus of the tick Ixodes ricinus (Acari: Ixodidae): a new insight into the mechanism of blood-feeding Scientific Reports 10: 165.
DOI: 10.1038/s41598-019-56811-2
Věchtová P., Fussy Z., Štěrba J., Erhart J., Beneš V., Grubhoffer L. (2020) Catalogue of stage-specifc transcripts in Ixodes ricinus and their potential functions during the tick life-cycle Parasites & Vectors 13: 311.
DOI: 10.1186/s13071-020-04173-4
Zubriková D. , Wittmann M., Hönig V., Švec P., Víchová B., Essbauer S., Dobler G., Grubhoffer L., Pfister K. (2020) Prevalence of tick-borne encephalitis virus and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in Lower Bavaria and Upper Palatinate, Germany Ticks and Tick-borne Diseases 11: 101375.
DOI: 10.1016/j.ttbdis.2020.101375
Hönig V., Švec P., Marek L., Mrkvička T., Zubrikova D., Wittmann M., Masař O., Szturcová D., Růžek D., Pfister K., Grubhoffer L. (2019) Model of Risk of Exposure to Lyme Borreliosis and Tick-Borne Encephalitis Virus-Infected Ticks in the Border Area of the Czech Republic (South Bohemia) and Germany (Lower Bavaria and Upper Palatinate) International Journal of Environmental Research and Public Health 16: 1173.
DOI: 10.3390/ijerph16071173
Rego R. O. M., Trentelman J.J., Anguita J., Nijhof A., Sprong H., Klempa B., Hajdušek O., Tomás-Corázar J., Azagi T., Strnad M., Knorr S. , Šíma R., Jalovecká M., Havlíková S., Ličková M., Sláviková M., Kopáček P., Grubhoffer L., Hovius J. (2019) Counterattacking the tick bite: towards a rational design of anti-tick vaccines targeting pathogen transmission Parasites & Vectors 12: 229.
DOI: 10.1186/s13071-019-3468-x
Selinger M., Tykalová H., Štěrba J., Věchtová P., Vavrušková Z., Lieskovská J., Kohl A., Schnettler E., Grubhoffer L. (2019) Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. Plos Neglected Tropical Diseases 27: e0007745.
DOI: 10.1371/journal.pntd.0007745
Švec P., Hönig V., Zubrikova D., Wittmann M., Pfister K., Grubhoffer L. (2019) The use of multi-criteria evaluation for the selection of study plots for monitoring of I. ricinus ticks - Example from Central Europe Ticks and Tick-borne Diseases 10: 905–910.
DOI: 10.1016/j.ttbdis.2019.04.014
Vancová M., Bílý T., Nebesářová J., Grubhoffer L., Bonnet S., Šimo L. (2019) Ultrastructural mapping of salivary gland innervation in the tick Ixodes ricinus Scientific Reports 9: 6860.
DOI: 10.1038/s41598-019-43284-6
Gerhart J.G, Dutcher H.A., Brenner A.E., Moses A.S., Grubhoffer L., Raghavan R. (2018) Multiple Acquisitions of Pathogen-Derived Francisella Endosymbionts in Soft Ticks. Genome Biology and Evolution 10: 607-615.
DOI: 10.1093/gbe/evy021
Müllerová J., Elsterová J., Černý J., Ditrich O., Žárský J., Culler L.E., Kampen H., Walther D., Coulson S., Růžek D., Grubhoffer L. (2018) No indication of arthropod-vectored viruses in mosquitoes (Diptera: Culicidae) collected on Greenland and Svalbard Polar Biology 11: 1581–1586.
DOI: 10.1007/s00300-017-2242-9

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