My research activity is the study of human viral infections, disease development and its treatment options.
Interest is focused on tick-borne encephalitis virus and related viruses transmitted by ticks and mosquitoes, as well as other viruses that require attention, such as SARS-CoV2.
Education
Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic
2012-2016
Ph.D. (hons.)
Infectious Biology
Tick-borne encephalitis – from pathogenesis to therapy
2015
RNDr.
Clinical Biology
Mice with different susceptibility to tick-borne encephalitis virus infection show selective neutralizing antibody response and inflammatory reaction in the central nervous system
2009-2012
MSc.
Clinical Biology
The role of the host genetic background on the pathogenesis of tick-borne encephalitis
2007-2009
2006-2009
BSc. (hons.)
Biology
Biology-Biomedical Laboratory Techniques
Study of the pathogenesis of tick-borne encephalitis in hosts with different genetic and immunological background
Employment
2014-present:
Researcher
Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic
2011-present:
Head of Lab
Laboratory of Arbovirology Institute of Parasitology, Biology Centre, Czech Academy of the Sciences
2011-2012:
Assistant
Laboratory of Biological Monitoring and Protection, The National Institute for Nuclear, Chemical and Biological Protection, Czech Republic
2009:
Assistant
Dept. of Parasite Immunology , Institute of Parasitology, Biology Centre, Czech Academy of the Sciences
Research Funding (PI)
2023
Neuropathogenesis linked to NS1 protein of tick-borne encephalitis virus; The Czech Science Foundation (GACR); (2023 - 2025)
2022
Verification of the possibility of using a mouse antibody against the protein of the tick-borne encephalitis virus for the purposes of diagnosis of tick-borne encephalitis by the ELISA method; Technology Agency of the Czech Republic GAMA2;
2020:
Neurovascular unit cells interactions leading to the blood-brain barrier breakdown during tick-borne encephalitis; The Czech Science Foundation (GACR); (2020 - 2022)
2020:
Identification and characterization of TBEV-neutralizing and cross-reactive antibodies from individuals that are convalescent from flaviviral infection; Programme for research and mobility support of starting researchers from Czech Academy of Sciences (2020-2021)
2017:
Programme of Support of Postdoctoral Fellowships from Czech Academy of Sciences (2017-2018)
2015:
Role of the human brain microvascular endothelial cells during tick-borne encephalitis virus infection in the central nervous system; Grant Agency of the University of South Bohemia
2014:
Role of the human non-neural cells in the neuronal injury during tick-borne encephalitis; Grant Agency of the University of South Bohemia in Ceske Budejovice
Postgraduate Courses and Internships
2020-2021:
Internship: Immunology and Infectious Disease (Davide Robbiani), Institute for Research in Biomedicine, Switzerland
2014:
PREDEMICS, National Institute for Infectious Diseases “L. Spallanzani” – Rome: Workshop on zoonotic features of viral infections from the animal reservoir to pandemic emergence
2012:
Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)
2011:
Erasmus Programme: Arbovirus Pathogenesis Group, The Roslin Institute and Royal (Dick)School of Veterinary Studies, The University of Edinburgh (4 mos.)
Honors
2021:
Prize by the President of Czech Academy of Sciences for young scientists for outstanding results
2018:
Otto Wichterle's Award for selected, exceptionally high-quality and promising scientists from the Czech Academy of Sciences
2017:
Dean’s Award for excellent scientific results during Ph.D. studies. Faculty of Science, University of South Bohemia
2015:
Award for the best Czech and Slovak young microbiologist of the year (Czechoslovak Society for Microbiology; XXIV. Tomasek Days of young microbiologists, Brno)
2013:
Best poster award (Moscow, “Chumakov IPVE” RAMS, Up-to-date problems of TBE)
15th International Symposium on Ticks and Tick-borne Diseases; Germany, Weimar (invited)
2022
10th Tick and Tick-Borne Pathogen Conference, Murighiol, Romania
2022
Konference: Národní institut virologie a bakteriologie, Czech Republic, Kutná Hora
2022
XXIV. Hradecké virologické dny, Czech Republic, Hradec Králové
2022
24th International Symposium on Signal Transduction at the Blood-Brain Barriers, Bari, Italy
2019:
22nd International Symposium on Signal Transduction at The Blood-Brain Barriers, Germany, Würzburg
2019:
The 67th Annual Meeting of the Japanese Society for Virology, Japan, Tokyo
2019:
Joint CzechoSlovak Virology Conference 2019 and 1st SK-AT Structural Virology Meeting, Bratislava
2018:
21nd International Symposium on Signal Transduction at the Blood Brain Barriers, Romania, Arad
2018:
28. Pečenkovy epidemiologické dny, České Budějovice
2017:
Tick and Tick-borne Pathogens 9 (One Health), Australia, Cairns
2017:
Joint CzechoSlovak Virology Conference 2017, České Budějovice
2015:
International Symposium on Flaviviruses: Structure and Immunity, Austria, Vienna
Selected applied research
0550809 - FZÚ 2022 RIV CZ cze L - Prototyp, funkční vzorek Antifoulingový povrch biočipu s terpolymerní vrstvou postmodifikovanou specifickou protilátkou pro kvantitativní stanovení viru SARS-CoV-2 v klinických vzorcích pomocí metody QCM.
0555327 - BC 2022 RIV cze P1 - Užitný vzor, průmyslový vzor Sada primerů pro amplifikační kontrolu pro detekci koronaviru SARS-CoV-2 metodou izotermické amplifikace LAMP a kit pro detekci koronaviru SARS-CoV-2 metodou izotermické amplifikace LAMP.
0542199 - FZÚ 2022 RIV CZ cze L - Prototyp, funkční vzorek Ultra-rezistentní povrch biočipu s terpolymerní vrstvou postmodifikovanou specifickou protilátkou pro rychlou detekci viru SARS-CoV-2 pomocí metody QCM.
0537039 - FZÚ 2021 RIV CZ cze L - Prototyp, funkční vzorek Funkční biočip s ultra-rezistentní polymerní vrstvou pro rychlou detekci viru SARS-CoV-2 pomocí metody QCM.
O - Ostatní výsledky, 2015, Detection of tick-borne encephalitis virus subtypes by RT-qPCR; Předkladatel: Státní ústav jaderné, chemické a biologické ochrany, v.v.i., Dodavatel: MV0 - Ministerstvo vnitra
O - Ostatní výsledky, 2015, Virus Serotyping by TaqMan and SYTO 9 in Single Reaction; Předkladatel: Státní ústav jaderné, chemické a biologické ochrany, v.v.i., Dodavatel: MV0 - Ministerstvo vnitra
O - Ostatní výsledky, 2014, Flavivirus Detection by Quantitative PCR; Předkladatel: Státní ústav jaderné, chemické a biologické ochrany, v.v.i., Dodavatel: MV0 - Ministerstvo vnitra
Other
Member of the International Brain Barriers Society (IBBS)
Member of the World Society for Virology (WSV)
Member of the Czechoslovak Society for Microbiology
Member of the Scientific Council of Institute of Parasitology, Biology Centre CAS
Certificate for the sex and gender dimension of biomedical research completed Libra course
Certificate for the performance of diagnostic methods and preparation of medications
(Institute for Postgraduate education in Medicine, Prague)
Certificate of competency according to § 15d of the Act No. 246/1992 coll. on Protection Animals against Cruelty in present statues at large. Czech University of Life Sciences in Prague, Central commission for Animal Welfare (CCAW)
Bianchini F., Crivelli V., Abernathy M.E., Guerra C., Palus M., Muri J., Marcotte H., Piralla A., Pedotti M. , de Gasparo R., Simonelli L., Matkovic M., Toscano C., Biggiogero M., Calvaruso V.,
Svoboda P., Cervantes Rincón T., Fava T., Podešvová L., Shanbhag A.A., Celoria A., Sgrignani J., Štefánik M.,
Hönig V.,
Prančlová V., Michalcikova T., Procházka J., Guerrini G., Mehn D., Ciabattini A., Abolhassani H., Jarrossay D., Uguccioni M., Medaglini D., Pan-Hammarström Q., Calzolai L., Fernandez D. , Baldanti F., Franzetti-Pellanda A., Garzoni C., Sedlacek R.,
Růžek D., Varani L., Cavalli A., Barnes C.O., Robbiani D.F. (2023) Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoproteinScience Immunology8: eade0958.
DOI: 10.1126/sciimmunol.ade0958
