HR Excellence in Science

RNDr. Martin Palus, Ph.D.

Position Head of Laboratory - Laboratory of Arbovirology
Tick-Borne Diseases
Institute of Parasitology

Contact details +420 387775145


Research interests

The researcher is interested in the study of human viral infections and the possibilities of their treatment.

The main interest is tick-borne encephalitis virus and related viruses transmitted by ticks and mosquitoes (Zika virus, West Nile fever, Chikungunya virus, Usutu virus, hemorrhagic fever viruses,...), but also other viruses that require attention, such as SARS-CoV2.

Specifically, for example, the study of the interactions of tick-borne encephalitis virus with the blood-brain barrier, neuronal damage, the inflammatory response triggered by the virus (immunopathology), antibody therapy,...


Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic


Ph.D. (hons.)

Infectious Biology

Tick-borne encephalitis – from pathogenesis to therapy



Clinical Biology

Mice with different susceptibility to tick-borne encephalitis virus infection show selective neutralizing antibody response and inflammatory reaction in the central nervous system



Clinical Biology

The role of the host genetic background on the pathogenesis of tick-borne encephalitis



BSc. (hons.)


Biology-Biomedical Laboratory Techniques

Study of the pathogenesis of tick-borne encephalitis in hosts with different genetic and immunological background




Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic


Head of Lab

Laboratory of Arbovirology Institute of  Parasitology, Biology Centre, Czech Academy of the Sciences



Laboratory of Biological Monitoring and Protection, The National Institute for Nuclear, Chemical and Biological Protection, Czech Republic



Dept. of Parasite Immunology , Institute of Parasitology, Biology Centre,  Czech Academy of the Sciences

Research Funding (PI)


Neurovascular unit cells interactions leading to the blood-brain barrier breakdown during tick-borne encephalitis; The Czech Science Foundation (GACR); 8 426 thousands of CZK; (2020 - 2022)


Identification and characterization of TBEV-neutralizing and cross-reactive antibodies from individuals that are convalescent from flaviviral infection; Programme for research and mobility support of starting researchers from Czech Academy of Sciences (2020-2021)


Programme of Support of Postdoctoral Fellowships from Czech Academy of Sciences (2017-2018)


Role of the human brain microvascular endothelial cells during tick-borne encephalitis virus infection in the central nervous system; Grant Agency of the University of South Bohemia


Role of the human non-neural cells in the neuronal injury during tick-borne encephalitis; Grant Agency of the University of South Bohemia in Ceske Budejovice

Postgraduate Courses and Internships


Internship: Immunology and Infectious Disease (Davide Robbiani), Institute for Research in Biomedicine, Switzerland


PREDEMICS, National Institute for Infectious Diseases “L. Spallanzani” – Rome: Workshop on zoonotic features of viral infections from the animal reservoir to pandemic emergence


Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)


Erasmus Programme:  Arbovirus Pathogenesis Group, The Roslin Institute and Royal (Dick)School of Veterinary Studies, The University of Edinburgh (4 mos.)



Prize by the President of Czech Academy of Sciences for young scientists for outstanding results


Otto Wichterle's Award for selected, exceptionally high-quality and promising scientists from the Czech Academy of Sciences


Dean’s Award for excellent scientific results during Ph.D. studies. Faculty of Science, University of South Bohemia


Award for the best Czech and Slovak young microbiologist of the year (Czechoslovak Society for Microbiology; XXIV. Tomasek Days of young microbiologists, Brno)


Best poster award (Moscow, “Chumakov IPVE” RAMS, Up-to-date problems of TBE)



ORCID ID: 0000-0002-4279-7092

WoS RID: N-1263-2017

Scopus Author ID=25825507600



Selected conferences contribution (talk/poster)


22nd International Symposium on Signal Transduction at The Blood-Brain Barriers, Germany, Würzburg


The 67th Annual Meeting of the Japanese Society for Virology, Japan, Tokyo


Joint CzechoSlovak Virology Conference 2019 and 1st SK-AT Structural Virology Meeting, Bratislava


21nd International Symposium on Signal Transduction at the Blood Brain Barriers, Romania, Arad


28. Pečenkovy epidemiologické dny, České Budějovice


Tick and Tick-borne Pathogens 9 (One Health), Australia, Cairns


Joint CzechoSlovak Virology Conference 2017, České Budějovice


International Symposium on Flaviviruses: Structure and Immunity, Austria, Vienna

Selected applied research

Prototype 2021: HL1/FZU/2021: Antifouling biochip surface based on terpolymer specific antibody-postmodified layer for rapid detection of SARS-CoV-2 virus by means of quartz crystal microbalance (QCM) method

Prototype 2021: Methodological platform for rapid  detection of SARS-CoV-2 based on loop mediated isothermal amplification

Prototype 2020: HL1/FZU/2020: Functional biochip based on ultra-low fouling polymer for rapid detection of SARS-COV-2 virus in crude biological fluids


Member of the Czechoslovak Society for Microbiology

Certificate for the performance of diagnostic methods and preparation of medications

(Institute for Postgraduate education in Medicine, Prague)

Certificate of competency according to § 15d of the Act No. 246/1992 coll. on Protection Animals against Cruelty in present statues at large. Czech University of Life Sciences in Prague, Central commission for Animal Welfare (CCAW)

Total found: 39 records
Bianchini F., Crivelli V., Abernathy M.E., Guerra C., Palus M., Muri J., Marcotte H., Piralla A., Pedotti M. , de Gasparo R., Simonelli L., Matkovic M., Toscano C., Biggiogero M., Calvaruso V., Svoboda P., Cervantes Rincón T., Fava T., Podešvová L., Shanbhag A.A., Celoria A., Sgrignani J., Štefánik M., Hönig V., Prančlová V., Michalcikova T., Procházka J., Guerrini G., Mehn D., Ciabattini A., Abolhassani H., Jarrossay D., Uguccioni M., Medaglini D., Pan-Hammarström Q., Calzolai L., Fernandez D. , Baldanti F., Franzetti-Pellanda A., Garzoni C., Sedlacek R., Růžek D., Varani L., Cavalli A., Barnes C.O., Robbiani D.F. (2023) Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein Science Immunology 8: eade0958.
DOI: 10.1126/sciimmunol.ade0958
Fořtová A., Hönig V., Salát J., Palus M., Pýchová M., Krbková L., Barkhash A.V., Kříha M.F., Chrdle A., Lipoldová M., Růžek D. (2023) Serum matrix metalloproteinase-9 (MMP-9) as a biomarker in paediatric and adult tick-borne encephalitis patients Virus Research 324: 199020.
DOI: 10.1016/j.virusres.2022.199020
Kevély Á., Prančlová V., Sláviková M., Haviernik J., Hönig V., Nováková E., Palus M., Růžek D., Klempa B., Koči J. (2023) Fitness of mCherry reporter tick-borne encephalitis virus in tick experimental models Viruses-Basel 14: 2673.
DOI: 10.3390/v14122673
Ribeiro J., Hartmann D., Bartošová-Sojková P., Debat H., Moos M., Fara J., Šimek P., Palus M., Kučera M., Hajdušek O., Sojka D., Kopáček P., Perner J. (2023) Blood-feeding adaptations and virome assessment of the poultry red mite Dermanyssus gallinae guided by RNA-seq Communications Biology 6: 517.
DOI: 10.1038/s42003-023-04907-x
Fořtová A., Hönig V., Palus M., Salát J., Pýchová M., Krbková L., Vyhlídalová T., Kříha M.F., Chrdle A., Růžek D. (2022) Serum and cerebrospinal fluid phosphorylated neurofilament heavy subunit as a marker of neuroaxonal damage in tick-borne encephalitis Journal of General Virology 103: 1743.
DOI: 10.1099/jgv.0.001743
Kovacech B., Fialova L., Filipcik P., Skrabana R., Zilkova M., Paulenka-Ivanovova N., Kovac A., Palova D., Paulikova Rolkova G., Tomkova K., Turic Csokova N., Markova K., Skrabanova M., Sinska K., Basheer N., Majerova P., Hanes J., Parrak V., Prcina M., Cehlar O., Cente M., Piestansky J., Fresser M., Novák M., Sláviková M., Borsova K., Cabanova V., Brejová B., Vinař T., Nosek J., Klempa B., Eyer L., Hönig V., Palus M., Růžek D., Vyhlídalová T., Straková P., Mrazkova B., Zudova D., Koubkova G,, Novosadova V., Procházka J., Sedlacek R., Zilka N., Kontsekova E. (2022) Monoclonal antibodies targeting two immunodominant epitopes on the Spike protein neutralize emerging SARS-CoV-2 variants of concern Ebiomedicine 76: 103818.
DOI: 10.1016/j.ebiom.2022.103818
Migné C.V., Hönig V., Bonnet S.I., Palus M., Rakotobe S., Galon C., Heckmann A., Výletová E., Devillers E., Attoui H., Růžek D., Moutailler S. (2022) Evaluation of two artificial infection methods of live ticks as tools for studying interactions between tick-borne viruses and their tick vectors Scientific Reports 12: 491.
DOI: 10.1038/s41598-021-04498-9
Agudelo M., Palus M., Keeffe J., Bianchini F., Svoboda P., Salát J., Peace A., Gazumyan A., Cipolla M., Kapoor T., Guidetti F., Yao K.H., Elsterová J., Teislerová D., Chrdle A., Hönig V., Oliveira T., West A.P., Lee Y.E., Rice C.M., MacDonald M.R., Bjorkman P.J., Růžek D., Robbiani D.F., Nussenzweig M.C. (2021) Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease. Journal of Experimental Medicine 218: e20210236.
DOI: 10.1084/jem.20210236
Beliavskaia A., Hönig V., Erhart J., Vyhlídalová T., Palus M., Černý J., Kozlova I., Růžek D., Palomar A.M., Bell-Sakyi L. (2021) Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. Frontiers in Veterinary Science 8: 659786.
DOI: 10.3389/fvets.2021.659786
de Gasparo R., Pedotti M., Simonelli L., Nickl P., Mueckschh F., Cassaniti I., Percivalle E., Lorenzi J.C.C., Mazzola F., Magrì D., Michalcikova T., Haviernik J., Hönig V., Mrazkova B., Polakova N., Fortova A., Tureckova J., Iatsiuk V., Di Girolamoa S., Palus M., Zudova D., Bednar P., Bukova I., Bianchini F., Mehni D., Nencka R., Straková P., Pavlis O., Rozman J., Gioria S., Sammartino J.C., Giardina F., Gaiarsak S., Hammarström Q.P., Barnes C.O., Bjorkmann P.J., Calzola L., Piralla A., Baldanti F., Nussenzweig M.C., Bieniasz P.D., Hatziioannouh T., Procházka J., Sedlacek R., Robbiani D.F., Růžek D., Varani L. (2021) Bispecific antibody neutralizes SARS-CoV-2 variants, protects mice from disease and prevents escape Nature 593: 424–428.
DOI: 10.1038/s41586-021-03461-y
Forinová M., Pilipenco A., Víšová I., Lynn N.S., Dostálek J., Mašková H., Hönig V., Palus M., Selinger M., Kočová P., Dycka F., Štěrba J., Houska M., Vrabcová M., Horak P., Anthi J., Tung C.P., Yu C.M., Chen C.Y., Huang Y.C., Tsai P.H., Lin S.Y., Hsu H.J., Yang A.S., Dejneka A., Vaisocherová-Lísalová H. (2021) Functionalized terpolymer-brush-based biointerface with improved antifouling properties for ultra-sensitive direct detection of virus in crude clinical samples ACS Applied Materials and Interfaces 50: 60612–60624.
DOI: 10.1021/acsami.1c16930
Haviernik J., Eyer L., Yoshii K., Kobayashi S., Černý J., Nougairède A., Driouich J.S., Volf J., Palus M., de Lamballerie X., Gould E., Růžek D. (2021) Development and characterization of recombinant tick-borne encephalitis virus expressing mCherry reporter protein: A new tool for high-throughput screening of antiviral compounds, and neutralizing antibody assays Antiviral Research 185: 104968.
DOI: 10.1016/j.antiviral.2020.104968
Petry M., Palus M., Leitzen E., Mitterreiter J.G., Huang B., Kröger A., Verjans G.M.G.M., Baumgärtner W., Rimmelzwaan G.F., Růžek D., Osterhaus A., Prajeeth C.K. (2021) Immunity to TBEV related flaviviruses with reduced pathogenicity protects mice from disease but not from TBEV entry into the CNS Vaccines 9: 196.
DOI: 10.3390/vaccines9030196
Salát J., Huňady M., Schanilec P., Straková P., Štefánik M., Svoboda P., Strelcova L., Bojcukova J., Palus M., Růžek D. (2021) Experimental and natural infections of tick-borne encephalitis virus in dogs Viruses-Basel 13: 2039.
DOI: 10.3390/v13102039
Haviernik J., Eyer L., Yoshii K., Kobayashi S., Černý J., Nougairède A., Driouich J.S., Volf J., Palus M., de Lamballerie X., Gould E., Růžek D. (2020) Development and characterization of recombinant tick-borne encephalitis virus expressing mCherry reporter protein: a new tool for high-throughput screening of antiviral compounds, and neutralizing antibody assays Antiviral Research 185: 104968.
DOI: 10.1016/j.antiviral.2020.104968
Helmová R., Hönig V., Tykalová H., Palus M., Bell-Sakyi L., Grubhoffer L. (2020) Tick-borne encephalitis virus adaptation in different host environments and existence of quasispecies Viruses 12: 902.
DOI: 10.3390/v12080902
Lattová E., Straková P., Pokorná Formanová P., Grubhoffer L., Bell-Sakyi L., Zdráhal Z., Palus M., Růžek D. (2020) Comprehensive N-glycosylation mapping of envelope glycoprotein from tick-borne encephalitis virus grown in human and tick cells Scientific Reports 10: 13204.
DOI: 10.1038/s41598-020-70082-2
Salát J., Mikulášek K., Larralde O., Pokorná Formanová P., Chrdle A., Haviernik J., Elsterová J., Teislerová D., Palus M., Eyer L., Zdráhal Z., Petrik J., Růžek D. (2020) Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and may Elicit NS1-Specific Antibody Responses in Vaccinated Individuals. Vaccines 8: 81.
DOI: 10.3390/vaccines8010081
Dufková P., Širmarová J., Salát J., Hönig V., Palus M., Růžek D., Fooks A., Mansfield K.L., Tordo N., Jochmans D., Neyts J., Byron M., Koraka P., Osterhaus A.D.M.E., Asklepios consortium (2019) Mannitol treatment is not effective in therapy of rabies virus infection in mice Vaccine 37: 4710–4714.
DOI: 10.1016/j.vaccine.2017.12.028
Hönig V., Palus M., Kašpar T., Majerová K., Zemanová M., Hofmannová L., Papežík P. , Šikutová S., Rettich F., Hubálek Z., Rudolf I., Votýpka J., Modrý D., Růžek D. (2019) Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019) Microorganisms 7: 568.
DOI: 10.3390/microorganisms7110568

Documents to download:
Honig_2019_Microorganisms (pdf)

More publications


Biology Centre CAS
Branišovská 1160/31
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Data box: r84nds8


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+420 387 775 051 (secretariat)
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